Thermostable Reverse Transcriptase

This reverse transcriptase is an RNA-dependent DNA polymerase used for real-time fluorescent RT-PCR with either SYBR Green dye or TaqMan probe. It originates from a type A DNA polymerase of thermophilic bacterium and works best at 60-62°C. The enzyme is easily heat-inactivated at ≥90°C for 1 minute and efficiently synthesizes complementary DNA on RNA template, reverse-transcribing single digit copies of target RNA molecules consistently. It is particularly suitable for one-step real-time qualitative and quantitative RT-PCR with preferable PCR product sizes of ≤150 bp.

Enzyme that synthesizes complementary DNA (cDNA) from RNA templates. Essential for RT-PCR and RNA-based molecular analysis applications.

DBscript Reverse Transcriptase is an enzyme reagent for RT-PCR and molecular diagnostic applications. It enables conversion of RNA to complementary DNA for downstream amplification in viral detection, gene expression analysis, and diagnostic testing workflows.

This DNA-dependent DNA polymerase is specially engineered for real-time PCR and RT-PCR with TaqMan probe in singleplex or multiplex formats. It is used together with Thermophilic Reverse Transcriptase in RT-PCR applications. The polymerase has increased 5'-3' exonuclease activity that generates an S-shaped fluorescence curve and is supplied with a 5x buffer available in three ROX concentration formulations.

This DNA-dependent DNA polymerase is used for real-time PCR and RT-PCR with SYBR Green dye. It is used together with Thermophilic Reverse Transcriptase for RT-PCR applications. The polymerase can extend more than 300 bases with short PCR cycling programs and is supplied with a 5x buffer available in three ROX concentration formulations.

ReverTra Ace -α- is a reverse transcriptase enzyme used in molecular diagnostics and RT-PCR applications for detecting RNA targets. This thermostable enzyme enables synthesis of cDNA from RNA templates with high fidelity and sensitivity, supporting viral detection and gene expression analysis in clinical and research laboratories.

A benchtop-stable, glycerol-free 2x master mix with a highly stringent hot-start Taq DNA polymerase and a 20x reverse transcriptase and RNase inhibitor enzyme mix optimized for multiplex target detection. It retains activity after incubation of fully assembled reactions at ambient temperature for at least eight hours and is ideal for high-throughput diagnostic workflows and detection of low-copy DNA and RNA pathogens. Available in pack sizes of 4 x 1.25 mL, 1 x 100 mL, and 10 x 100 mL at 2x concentration.

This master mix is used for real-time qualitative and quantitative RT-PCR amplifications with TaqMan probe in singleplex format. The premixed, 2x concentrated solution contains all components except gene-specific primers, probe, and RNA template. It uses Thermophilic Reverse Transcriptase and Taq-Probe DNA polymerase specially engineered for TaqMan probe to generate S-shaped fluorescence curves with a preferable PCR product size of ≤150 bp.

This master mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye, particularly for fast thermocycling in singleplex format. The premixed, 2x concentrated solution contains all components except gene-specific primers and RNA template. It uses Thermophilic Reverse Transcriptase and standard Taq DNA polymerase with a preferable PCR product size of ≤150 bp.

This master mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye, particularly for fast thermocycling in singleplex format. The premixed, 2x concentrated solution contains all components except gene-specific primers and RNA template. It uses Thermophilic Reverse Transcriptase and Taq-Fast DNA polymerase for enhanced speed and efficiency with a preferable PCR product size of ≤150 bp.

Thermostable DNA polymerase enzyme essential for PCR applications. Enables efficient DNA synthesis and amplification in molecular biology procedures.

An advanced thermal cycler for real-time quantitative PCR (qPCR) applications in life science research. This instrument enables real-time monitoring of DNA amplification during the PCR process for quantitative nucleic acid analysis.